Biochemical studies on bovine adenovirus type 3. I. Purification and properties
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چکیده
منابع مشابه
Biochemical Studies of Virus Reproduction I. Purification and Properties of Escherichia Coli
Bacteriophages possess the fundamental properties of viruses, yet exhibit ease of assay, handling, and culture, and thus present suitable systems for isotope tracer studies of the mode of virus reproduction and protein synthesis. In an investigation of this problem we have employed the Escherichia coli bacteriophage Ta system. This communication outlines methods for the growth and isolation of ...
متن کاملEffect of bovine adenovirus 3 on mitochondria
Viruses alter the structure and the function of mitochondria for survival. Electron microscopy analysis of the cells infected with bovine adenovirus 3 revealed extensive damage to the inner mitochondrial membrane characterized by dissolution of the cristae and amorphous appearance of mitochondrial matrix with little or no damage to the outer mitochondrial membrane. There were fewer cristae with...
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The sporulation-specific enterotoxin of Clostridium perfringens type A, which is the toxin active in human food poisoning, has been purified from extracts of sporulating cells. Highly purified enterotoxin was obtained by treatment of crude cell extract with ribonuclease for 30 min, followed by sequential chromatography on Sephadex G-100, Cellex T cellulose, and hydroxylapatite. Recovery was 65 ...
متن کاملReplication-defective bovine adenovirus type 3 as an expression vector.
Although recombinant human adenovirus (HAV)-based vectors offer several advantages for somatic gene therapy and vaccination over other viral vectors, it would be desirable to develop alternative vectors with prolonged expression and decreased toxicity. Toward this objective, a replication-defective bovine adenovirus type 3 (BAV-3) was developed as an expression vector. Bovine cell lines designa...
متن کاملDevelopment of a bovine adenovirus type 3-based expression vector.
We constructed a non-defective bovine adenovirus type 3 recombinant (BAd3-Luc) containing the firefly luciferase gene inserted in the early region 3 (E3) of the BAd3 genome. Deletion of a 696 bp XhoI-NcoI E3 segment and insertion of the luciferase gene in E3 was confirmed by Southern blot analyses. Luciferase was expressed in Madin-Darby bovine kidney cells infected with BAd3-Luc as measured by...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1975
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.16.3.621-633.1975